AMSI BioInfoSummer 2016 was held in November at the University of Adelaide, hosted by the School of Mathematical Sciences, with a collaborative effort in organisation from the University of Adelaide, University of South Australia, Flinders University and the South Australian Health and Medical Research Institute (SAHMRI). As one of AMSI’s flagship higher‐education events, AMSI BioInfoSummer provided bioinformatics training to students, researchers and academics alike, offering something for everyone by aiming to increase all delegates’ knowledge and skills in this truly interdisciplinary field. The symposium was opened by Professor Warren Bebbington (Vice‐Chancellor and President, The University of Adelaide), Leanne Harvey (A/g Chief Executive Officer, Australian Research Council) and Professor Terry Speed (Laboratory Head, Walter & Eliza Hall Institute of Medical Research and Chair of the AMSI Scientific Advisory Committee) in the Flentje Lecture Theatre, each addressing the delegation of over 200 with an inviting welcome to the University of Adelaide, AMSI BioInfoSummer and the comprehensive bioinformatics program ahead. The five‐day program, designed for mathematicians, statisticians and biologists, started with an introductory overview on day one followed by four days of specialised content focused on high‐dimensional data and multivariate analysis, experimental design and analysis for RNA sequencing, programming for bioinformatics and analysis of long‐read (third‐generation) data. Each day included a mix of keynote speakers, lectures and hands‐on workshop practical sessions, in addition to a variety of program extras.